Kadar MDA (Malondialdehyde) dan Aktivitas SOD (Superoxide Dismutase) pada Tikus Defisiensi Vitamin D dan Kalsium
Malondialdehyde (MDA) Levels and SOD (Superoxide Dismutase) Activity in Vitamin D and Calcium Deficiency Rat
DOI:
https://doi.org/10.35473/jgk.v17i1.732Abstract
Vitamin D and calcium deficiencies are often associated with increased oxidative stress. Oxidative stress conditions can cause cell damage and metabolic stress and induce degenerative diseases. Several parameters that are often used to measure oxidative stress are MDA (malondialdehyde) and SOD (superoxide dismutase) which can be found in blood or body tissue. This study aims to determine MDA levels and SOD activity in the livers of mice with vitamin D and calcium deficiency. This research used a pre-experimental design with a static group comparison design. A total of 12 male Wistar white rats (Rattus novergicus) were divided into two groups. The first group (K+) is a healthy control group without any treatment and the second group (K-) is a deficiency group given feed without vitamin D and calcium content (Modified AIN-93M (vitamin D and calcium depleted)). After 14 days of treatment, the MDA levels and SOD activity in the liver tissue were seen. MDA levels were measured using TBARS (Thiobarbituric Acid Reactive Substances) and SOD activity was measured using the Superoxide Dismutase (SOD) Activity Assay Kit. The results showed that MDA levels in the deficiency group (K-) were higher than in the control group (p<0.001) and SOD activity was lower than in the control group (p<0.001). MDA levels were higher and SOD activity was lower in the group of deficient mice (K-) compared to the group of healthy control mice (K+). Vitamin D and calcium deficiency conditions increase oxidative stress conditions in the liver of rat.
ABSTRAK
Defisiensi vitamin D dan kalsium sering dihubungkan dengan peningkatan stress oksidatif. Kondisi stres oksidatif dapat menyebabkan kerusakan sel dan stress metabolik dapat menginduksi penyakit degenerative. Beberapa parameter yang sering digunakan untuk mengukur stres oksidatif yaitu MDA (malondialdehyde) dan SOD (superoxide dismutase) yang bisa ditemukan di darah atau jaringan tubuh. Penelitian ini bertujuan untuk mengetahui kadar MDA dan aktivitas SOD pada organ hati tikus dengan defisiensi vitamin D dan kalsium. Penelitian ini menggunakan rancangan pra-eksperimental (pre-experimental) dengan design static group comparison. Sebanyak 12 ekor tikus putih (Rattus novergicus) jantan galur Wistar dibagi menjadi dua kelompok. Kelompok pertama (K+) yaitu kelompok kontrol sehat tanpa diberikan perlakuan apapun dan kelompok kedua (K-) yaitu kelompok defisiensi dengan diberikan pakan tanpa kandungan vitamin D dan kalsium (AIN-93M Termodifikasi (vitamin D and calcium depleted)). Setelah 14 hari perlakuan, dilihat kadar MDA dan aktivitas SOD di jaringan organ hati. Pengukuran kadar MDA menggunakan TBARS (Thiobarbituric acid reactive substances) dan pengukuran aktivitas SOD dengan menggunakan Superoxide Dismutase (SOD) Activity Assay Kit. Hasil penelitian menunjukkan kadar MDA pada kelompok defisiensi (K-) lebih tinggi dibandingkan kelompok kontrol (p< 0.001) dan aktivitas SOD lebih rendah dibandingkan kelompok kontrol (p<0.001). Kadar MDA lebih tinggi dan aktivitas SOD lebih rendah pada kelompok tikus defisieni (K-) dibandingkan kelompok tikus kontrol sehat (K+). Kondisi defisiensi vitamin D dan kalsium meningkatkan kondisi stres oksidatif pada organ hati tikus.
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